Evaluation of influence of salivary contamination during different application stages of 3 adhesive systems

Objectives: The aim of this study was to investigate the influence of salivary contamination on bond strength to dentin during different application stages of 3 different adhesive systems Materials and Methods: Twenty-four caries-free human molars were sectioned horizontally with a diamond saw to expose 1 mm thick dentin disks. Totally 48 disks (two dentin disks from each tooth) were obtained and these disks were randomly divided into 3 groups according to adhesives used (Prime Bond NT, Clearfil SE Bond, Clearfil S3 Bond). Each adhesive group was further subdivided into 4 groups according to contamination methods as follows: no contamination which was the control group, contamination before adhesive application, contamination after adhesive application and contamination after polymerization of the adhesive. Transparent plastic cylindrical molds with an internal diameter of 0.7 mm and a height of 1 mm were used to place composite resin on dentin disks in order to test the microshear bond strength of the groups Three resin cylinders were attached on each dentin surface and 12 specimens were made for each group. Data were analyzed by one way ANOVA and Tukey’s post hoc test. Results: Except when contamination occurred after the polymerization of two step self-etch adhesive, bond strength values of all groups showed statistically significant reduction compared with control groups (p<0.05). Conclusions: Within the limitations of this study, it can be concluded that, saliva contamination affects the bond strength to dentin of all adhesives used in this study and the type of adhesive and the stage of contamination can be effective on the bond strength of adhesive contaminated with saliva. ARTICLE INFO