Istraživanje enzima prikladnih za razgradnju lignina

Enzymatic degradation of kraft lignin was investigated using purified fungal enzymes known to degrade lignin structures. The activity of laccase, the most frequent enzyme associated with ligninolysis and the activity of auxiliary redox enzymes such as glucose dehydrogenase (GDH), cellobiose dehydrogenase (CDH) and lytic polysaccharide monooxygenase (LPMO) were tested. The effectiveness of these purified enzymes was compared with crude extracts obtained from fungal cultivations of the ligninolytic model fungi: Trametes versicolor and Phanerochaete chrysosporium. Laccase was proven to be the most efficient enzyme for lignin degradation. Auxiliary enzymes cooperate with other enzymes in order to enhance depolymerisation of lignocellulose. With observed high laccase activity, Trametes versicolor is found to be efficient in lignin degradation.