Rhizobium wenxiniae sp. nov., an endophytic bacterium isolated from maize root
2017
A novel Gram-stain-negative, aerobic, rod-shaped strain designated 166T was isolated from surface-sterilized root tissue of maize planted in the Fangshan District of Beijing, PR China. The 16S rRNA gene sequence analysis indicated that strain 166T belongs to the genus Rhizobium and is closely related to
Rhizobium cellulosilyticum
ALA10B2T and
Rhizobium yantingense
H66T with sequence similarities of 98.8 and 98.3 %, respectively. According to atp
D and rec
A sequence analysis, the highest sequence similarity between strain 166T and
R. cellulosilyticum
ALA10B2T is 93.8 and 84.7 %, respectively. However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to
R. cellulosilyticum
DSM 18291T (20.8±2.3 %) and
Rhizobium yantingense
CCTCC AB 2014007T (47.2±1.4 %). The DNA G+C content of strain 166T was 59.8 mol%. The main polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an unidentified aminophospholipid and an unidentified aminolipid. The major fatty acids of strain 166T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c). The results of the physiological and biochemical tests and minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 166T from the type strains of closely related species,
R. cellulosilyticum
DSM 18291T and R. yantingense CCTCC AB 2014007T. Strain 166T represents a novel species within the genus
Rhizobium
, for which the name Rhizobium wenxiniae sp. nov. is proposed, with the type strain 166T (=CGMCC 1.15279T=DSM 100734T).
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