Epinephrine and Adrenocorticotropic Hormone-stimulated Magnesium Accumulation in Adipocytes and Their Plasma Membranes

Abstract Epinephrine, norepinephrine, and adrenocorticotropic hormone stimulate magnesium accumulation in plasma membrane vesicles derived from fat cells. A new method was developed to demonstrate this magnesium uptake utilizing the ligand, 8-hydroxyquinoline-5-sulfonic acid, whose magnesium complex fluoresces distinctively. An increase in total magnesium content in whole fat cells following hormone stimulation was documented. These findings were confirmed with 28Mg2+ in intact adipocytes.