Análisis de degradación de stents metálicos en modelo de cultivo “in vitro” con células epiteliales respiratorias y fibroblastos

espanolOBJETIVO Investigar la posible degradacion de stents metalicos tras co-cultivo con celulas respiratorias in vitro. METODOLOGIA Durante 21 dias se han co-cultivado con la linea CRL-4011 (epitelial) y MRC-5 (fibroblastos) tres tipos de stents: Wallstent® (aleacion de cobalto-cromo-niquel y molibdeno), Zilver PTX® y Zilver Flex® (nitinol, aleacion de niquel-titanio, con y sin liberacion de paclitaxel, respectivamente). Las mismas celulas sin stent sirvieron como control. Los sobrenadantes de los cultivos se recogieron los dias 3, 9, 15 y 21, se alicuotaron y almacenaron a -800 C. Mediante espectrometria de masas (ICP/MS) se investigaron los niveles de titanio, cromo, niquel, cobalto y molibdeno en los sobrenadantes, y tambien se han analizado los niveles de esos mismos elementos en el medio de cultivo original (antes de anadirlo a los cultivos celulares). RESULTADOS En todos los experimentos se encontraron mayores niveles de elementos metalicos en los sobrenadantes recogidos en el tercer dia de cultivo, tanto de celulas epiteliales como de fibroblastos, con diferencias estadisticamente significativas (p CONCLUSIONES Hemos detectado una rapida liberacion en el sobrenadante de todos los cultivos de los elementos constitutivos de los tres stents que incluimos en los experimentos, y con niveles muy superiores a los cultivos controles. EnglishObjective: To investigate the possible degradation of metal stents after co-culture with respiratory cells in vitro. Methods: Three types of stents were co-cultured with the CRL-4011 line (epithelial) and the MRC-5 line (fibroblasts): Wallstent® (cobalt-chromium-nickelmolybdenum alloy), Zilver PTX® and Zilver Flex® (nitinol, nickel-titanium alloy, with and without paclitaxel release, respectively). The same stentless cells served as the control group. Culture supernatants were collected on days 3, 9, 15 and 21, aliquoted and stored at -80 oC. The levels of titanium, chromium, nickel, cobalt and molybdenum in the supernatants were studied using inductively coupled plasma mass spectrometry (ICP-MS), and the levels of the same elements were also analyzed in the original culture medium (before adding them to the cell cultures). Results: In all experiments, higher levels of metal elements were found in the supernatants collected on the third day of culture, for both epithelial cells and fibroblasts, with statistically significant differences (p Conclusion: We have detected a rapid release in the supernatant of all the cultures of the constituent elements of the three stents that we included in the experiments, and with levels much higher than those of the control cultures.