LIPID TRANSFER TO HDL IN TYPE 1 DIABETIC WOMEN

Introduction: despite normal or elevated levels in type 1 diabetic women, cardiovascular disease is the principal cause of death in this population. We aimed to study the HDL ability of subjects with type 1 diabetes mellitus receive lipids, such as free cholesterol, cholesteryl esters, phospholipids, and triglycerides from donor lipoproteins. Methods: twenty-one female subjects with type 1 diabetes mellitus on intensively insulin therapy and twenty-one non-diabetic women were studied. Two sets of nanoemulsion preparations were used as lipid donor to HDL: one labeled with 3H-triglycerides and 14C-free cholesterol and the other with 3H-cholesteryl esters and 14C-phospholipids was incubated with plasma samples for 1h. After chemical precipitation, the supernatant containing HDL was counted for radioactivity. HDL size was measured by laser-light-scattering. Results: Serum total cholesterol, apo Al, HDLc and HDL size did not differ between diabetics and non-diabetic subjects. Glycated hemoglobin levels in diabetic were 8.8±1.3%. In the similar way there was no difference in lipid transfer to HDL of cholesteryl esters, triglycerides, free cholesterol and phospholipids. Glycated hemoglobin and total daily insulin dose cause no influence in lipid transfer to HDL. Conclusion: the HDL ability of subjects with type 1 diabetes mellitus simultaneously receive lipids is preserved despite non ideal glycemic control in patient under intensive insulin therapy.