Abstract 2364: Long non-coding RNA HOTAIR is expressed in small cell lung cancer and relevant to cellular proliferation, invasiveness and clinical relapse - analyses based on tumor tissues and cell lines.

Small cell lung cancer (SCLC) is a subtype of lung cancer with poor prognosis, showing early relapse after initial fair response to chemotherapy. A long non-coding RNA, Hox transcript antisense intergenic RNA (HOTAIR) has been reported to be involved in breast and colon cancer metastasis. Here we revealed, using 35 surgical SCLC cases, that HOTAIR was expressed highly in pure, rather than combined, SCLC (p=0.012) and that a subset of SCLC with its higher expression tended to relapse earlier (p=0.086). Also, knock down of HOTAIR by siRNA transfection in an SCLC cell line, SBC-3, led to the reduced proliferation activity and invasiveness, due to changes of expression of cell adhesion-related genes. These findings suggest that the HOTAIR expression changes gene expression in relation to cell adhesion and subsequently alters proliferation activity and invasiveness of tumor cells, resulting in early relapse of SCLC. Our results imply that HOTAIR could be a prognostic biomarker and a therapeutic target of SCLC. Citation Format: Hiroshi Ono, Eisaku Miyauchi, Noriko Motoi, Masaru Ushijima, Masaaki Matsuura, Sakae Okumura, Makoto Nishio, Tetsuro Hirose, Satoru Itou, Mutsunori Fujiwara, Naohiko Inase, Yuichi Ishikawa. Long non-coding RNA HOTAIR is expressed in small cell lung cancer and relevant to cellular proliferation, invasiveness and clinical relapse - analyses based on tumor tissues and cell lines. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 2364. doi:10.1158/1538-7445.AM2013-2364