Tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense

The present invention relates to tissue culture propagation of Epimedium wushanense breeding methods and Epimedium wushanense. The method comprises the following steps: A the seeds were sterilized after Epimedium wushanense 0 ~ 10 ℃ low temperature stratification in the Sand; B after the lamination process seed disinfection, with sterile forceps Embryo stripping. a standby;. C the sterilized seed embryo induction medium the culture was inoculated in the seed embryo callus and adventitious buds or embryos directly induces the seed germination; D obtained in step C buds turn. into the growth medium, to generate tissue culture buds; step D E induce differentiation bud burst grown into plantlets rooting medium and cultured to form a white fibrous, thus forming a complete plant. This method can improve the propagation coefficient Wushan epimedium, shorten the breeding cycle, which is valuable for the protection of wild Wushan epimedium resources and promote their sustainable use and industrialization.