Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose

Clostidium stercorarium xylanase A (XynA) composed of a family 11 catalytic domain of glycosyl hydrolases and family VI CBDs bound to amorphous cellulose, i.e., acid-swollen cellulose (ASC), but not highly crystalline cellulose, and it was released from the cellulose protein complex by wash with a cellobiose solution. The Ka and [PC]max values of ASC were 0.25 liter/μmol and 26μmol/g.