Engelhardtia chrysolepis의 라디칼 소거능 및 신경세포의 산화 스트레스 보호효과

In this study, the radical scavenging activity and protective effect of ethanol extract from leaf of Engelhardtia chrysolepis HANCE (ECE) against oxidative stress were investigated under in vitro and cellular system. ECE showed strong radical scavenging activities in 1,1-diphenyl-2-picrylhydrazyl, hydroxyl(·OH) and nitric oxide(NO) radical as a concentration-dependent manner. Particularly, strong scavenging activity against the ·OH and NO radical were observed with the IC50 value of 1.30 μg/ml and 12.61 μg/ml, respectively. Furthermore, the cellular oxidative stress was induced by amyloid beta(Aβ25-35) in C6 glial cells. The treatment of Aβ25-35 to C6 glial cells generated high levels of reactive oxygen specie (ROS) and declined cell viability. However, production of ROS was decreased by the treatment of ECE. In addition, the cell viability was significantly increased at each concentration(10, 25, 50 μg/ml) as dose-dependent manner. The Alzheimer’s disease related protein expressions in Aβ25-35-treated C6 glial cells were analyzed. The ECE treatment inhibited expression of amyloid precursor protein(APP), Cterminal fragment-β(CTF-β), β-site APP cleaving enzyme(BACE), phosphorylated tau(p-tau) proteins in C6 glial cells induced by Aβ25-35. The present study indicated that ECE has strong radical scavenging activity and neuroprotective effect through attenuating oxidative stress.