THE ANTISPASMODIC EFFECT OF METANOLIC FRACTION DERIVED FROM TRACHYSPERMUM AMMIESSENSE ON RAT'S ILEUM CONTRACTION

Background and Objective: Since past time, herbal plants have been used for the treatment of different abnormalities such as gastrointestinal disorders. One of these herbs which used in Iranian traditional medicine is Trachyspermum ammi. There are many biological studies conducted for the determination of its pharmacological properties. The present study was designed to find out the effects of methanolic fraction derived from Trachyspermum ammi essense on mechanical activity of the isolated rat’s ileum. Materials and Methods: In this laboratory study, for evaluation of spasmolythic property of fraction, different doses of the solution were added to organ bath after acetylcholine with concentration of 10-4 molar (M) and for assesment of antispasmodic effect of fraction, different doses of the solution were added to the organ bath before acetylcholine with concentration of 10-9 upto 10-2 M, then isotonic contraction of ileum were recorded through an isolated tissue chamber in an organ bath by using isotonic transducer and oscillographic device. The results were compared with thymol as a positive control. Results: Our findings showed that different concentration of metanolic fraction of Trachyspermum ammi 0.002, 0.005 & 0.01% on acetylcholine 10-4 M induced contraction in rat’s ileum resulted in reduced contraction by the essense from concentration of 0.002% and higher. So that the concentration of 0.002% was reduced to 59.8% (P less-than  0.05) and concentration of 0.01% was reduced to 77.5% (P less-than  0.01). in addition thymol in concentration of 10-5 M & 10-4 M as compared with metanolic fraction of 0.002, 0.005 ; 0.01% had no significant effect on acetylcholine induced contraction. Conclusion: Methanolic fraction derived from Trachyspermum ammi essense has spasmolythic and antispasmodic property. Key word: Metanolic fraction, Trachyspermum ammi. Anti spasmodic, Acetylcholine, Ileum How to cite this article: Hejazian SH, Bagheri SM, Zare Zadeh A. The Antispasmodic effect of Metanolic Fraction derived from Trachyspermum ammi essense on Rat's ileum contraction. J RafsanjanUniv Med Sci 2014 13(2): 175-82. [Farsi]