[IL-1β can promote proliferation and migration of Hepa1-6 cells and impair IFNα-induced cell growth inhibition in vitro].

AIM:To analyze the effect of recombinant IL-1β on proliferation,migration,and the effect on IFNα induced cell growth inhibtion.METHODS:The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1.Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA.Cell migration was assessed using wound healing assay.RESULTS:IL-1β significantly stimulated proliferation and migration of Hepa1-6 cells.However,expression of IL-1β significantly down-regulated growth inhibition inducecd by IFNα.CONCLUSION:The recombinant vector could stably express IL-1β and promote in vitro proliferation,migration,and impair IFNα-induced cell growth inhibition.