Mechanisms of disruption of mitochondrial transport of adenine nucleotides in the course of acute hepatic ischemia

: Suppression of mitochondrial transport of adenine nucleotides (AN) in the ischemized liver is caused by inhibition of adenine nucleotide translocase by long chain acyl-KoA and by reduced exchange fund of intramitochondrial AN. It preceeds the disunion of oxidizing phosphorylation which develops during the period of marked structural disturbances of the mitochondrial membrane. The preincubation of mitochondria from the ischemized liver with carnitine and alpha-ketoglutarate stimulates breathing in the metabolic state. The ischemic toxin blocking SH-groups of the membrane proteins also inhibits the transport of AN. However, its effect on the mitochondrial membrane is dominated by the disintegrating component.