Field-Flow Fractionation for Assessing Biomolecular Interactions in Solution

Many biological systems are primarily governed by protein-protein interactions. It is important to develop sensitive analytical techniques to identify and characterize these bimolecular interactions in order to understand their fundamental roles in biological processes and in disease. In this book chapter, we summarize three case studies that applied asymmetrical flow field-flow fractionation (AF4) to access the protein-protein interactions of therapeutic proteins with their counterparts. These new applications of AF4 provide a unique and innovative tool that extends the bioanalytical capability to study protein complexes beyond micro-molar affinity.