Effect of 3-deoxy-3-fluoro-d-glucose on glycolytic intermediates and adenine nucleotides in resting cells of Saccharomyces cerevisiae

Abstract The effects of 3-deoxy-3-fluoro- d -glucose (3FG ∗ ) upon the levels of glycolytic intermediates, adenine nucleotides and inorganic phosphate in resting cell suspensions of Saccharomyces cerevisiae have been investigated. Significant decreases in the intracellular levels of UDPG, PGAs, ATP and inorganic phosphate were detected whilst increases in ADP, G-1-P and F-6-P were observed. The changes in ATP, ADP and G-1-P only became apparent after the changes in the other intermediates had occurred. It is argued that 3FG acts at least in part as a phosphate trap thereby occasioning a shift in the energy balance of these cells.