Определение циклосерина в плазме крови с использованием ВЭЖХ/МС: применение в исследовании биоэквивалентности

The method of assay for a new antituberculosis drug cycloserine in human blood plasma by high performance liquid chromatography (HPLC) with mass-spectrometric (MS) detection has been developed and validated. The proposed method selective, specific, accurate, precise, and linear in a range from 0.5 to 20.0 μg/mL. The calibration curve is described by the equation Y = aX + b , where a = 0.0893, b = –0.0134, and the correlation coefficient is 0.9994. The limit of detection (LOD) is 0.1 ng/mL and the limit of quantification (LOQ) is 0.5 μg/mL. The recovery of cycloserine determined at low (0.5 μg/mL) and high (20.0 μg/mL) drug concentrations was 41.7 and 62.4%, respectively; the matrix factor normalized to the internal standard was 0.28 and 0.34, respectively.