CONCENTRATION OF C-REACTIVE PROTEIN IN WHITE ADIPOSE TISSUE, LIVER AND BLOOD SERUM OF MALE WISTAR RATS

PURPOSE: The aim of the present study is to make a quantitative analyze of C-reactive protein (CRP) concentration in the white adipose tissue, liver and blood serum in experimentally induced obesity in rats. METHODS: A model of animal obesity was created using high-fat diet. Forty, eight-weeks-old male Wistar rats were randomly divided into two groups – 1) normal, control group fed with standard rodent food and 2) experimental group fed with a high-fat diet. They were subjected on these regiments for fourteen weeks. Blood serum, liver and adipose tissue specimens were obtained from each animal at the end of the experimental period. The concentration of CRP in all samples was quantified by ELISA method. RESULTS: Serum CRP concentration was elevated in the group with obesity, med.947.51 (95% CI 868.09 – 1049.93) μg/ml compared with the control group med.649.34 (95% 558.02 – 688.45) μg/ml, p<0.0001. The liver CRP concentration in obese animals was lower than the concentration in control animals, p=0.048. The concentration of CRP in adipose tissue doesn’t show any significant deference among the obese and control animals, p=0.826. CONCLUSION: The liver is an active participant in the development of low-grade, systemic inflammation in obese male rats, rather than adipose tissue.