[Effect of G-CSF on the proliferation and differentiation of bcr/abl(+)-CD34+ cells from CML patients].

Objective To study the effect of granulocyte colony-stimulating factor(G-CSF)on the proliferation and differentiation of hcr/abl~+ -CD34~+ cells.Methods bcr/abl~+-CD34~+ cells were isolated from bone marrow of chronic myelocytic leukemia(CML)patients and were treated with 0,10,100,1000 ng/ml of G-CSF fur 48,96,144 hs.CD34~+ cells from normal bone marrow were used as controls.Cell prolif- eration was determined by trypan blue dye exclusion,cell-cycle and antigen differentiation were determined by flow cylometry and cell morphology was observed under light microscope.Results The number of bcr/abl~+- CD34~+ cells was increased obviously in all groups.After cuhurcd for 48 and 96 h,the number of bcr/abl~+ - CD34~+ cells at G-CSF 10 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group(P 0.05),the number of normal CD34~+ cells was increased only in the presence of G-CSF.After cultured for 48,96 and 144 h,the cell number in G-CSF 100 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group(P0.05,P0.01 ,P0.01,respectively).After cultured for 144 h,the cell percentages in G_0/G_1 phase for bcr/abl~+ -CD34~+ cells in G-CSF 10,100,1000 ng/ml groups were significantly less than thai in G-CSF 0 ng/ml group(P0.05),and that for normal CD34~+cells in G-CSF 10,100,1000 ng/ml groups were significantly less than that of G-CSF 0 ng/nd group after cultured for 48 and 96 h.The expres- sions of CD34 on bcr/abl~+- CD34~+ cells and normal CD34~+ cells were decreased along with the cuhure dura- tinn,accompanied by the expression of CD33 and CD13 increased first and decreased later,which was not correlated with the concentration of G-CSF.Both bcr/abl~+ -CD34~+ cells and normal CD34~+ cells showed ma- ture morphology along with proliferation and differentiation.Conclusions G-CSF promotes proliferation of both bcr/abl~+ -CD34~+ cells and normal CD34~+ cells,but not necessary for the former,and the former differ- entiates more rapidly than the latter does,but both was independent of G-CSF.