Improvement of growth and proliferation of SP2/0 cell by natural-nanomaterial attapulgite.

Objective: To study the effect natural nanomaterial attapulgite (ATP) on the growth and proliferation of Sp2/0 cell,and whether this effect is related to the optimized cultivation circumstance by ATP.Methods: Adopting CCK-8 methods and cell counter,Sp2/0 cell viability and cell density in normal and ATP-assisted cultivation are measured.In addition,concentration of glutamine and ammonium in cell medium was measured,respectively.Results: Under some concentration range,the ATP can improve the Sp2/0 cell viability and cell density to some degree.Conclusion: ATP enhances the growth and proliferation of Sp2/0 cell by optimizing its culture environment.