Experimental study on the effects of artificial bone composite materials on bone formation

Objective To explore the osteogenic effect of collagen/hydroxyapatite(CHA) composite of bone morphogenetio protein(BMP) material in vitro and in repairing rabbit radius defect effect.Methods With the technique of cell culture in vitro,the human embryo osteoblast was cultured in vitro with HA,CHA,HA-BMP and CHA-BMP materials respectively and was observed the cell growth in the materials.The cell proliferation rate and secretion of ALP and OC were detected in cell frozen solution.The ability to induce ectopic bone was evaluated in the implant of mouse femoral muscle pouches.The laws of new bone formation were observed in the implantation of rabbit radius defect site after 2,4,8,16,24 weeks.Results Cell proliferation rate and the ALP and OC levels of CHA-BMP were significantly higher than the corresponding single material.Ectopic cartilage and bone tissue were induced visible and ALP was positive.X light displayed that bone stump formed dense fiber connection with a small amount of callus formation after 8 and 16 weeks.A lot of mature lamellar bone and marrow tissue were filled in the microscope.The immunohistochemistry of BMP,type I collagen and collagen were positive,and there were not bone or cartilage formation in non-BMP composites.Conclusions CHA is beneficial to early adhesion of bone cells,bone morphogenetic protein composite growth,mouse heterotopic bone induction and the acceleration of new bone formation in rabbit radius defect,which is an ideal material for bone repair.