Endogenous glutathione as potential protectant against free radicals in the skin of vitamin A deficient mice

Abstract In order to evaluate the relationships between oxidative degradation of lipids and antioxidant defence systems in the skin, weanling female SKH1 hairless mice were randomly divided into two groups. Each group was fed a well-balanced diet, supplemented, in one group with 5 IU vitamin A/g, and vitamin A free in the other, for 20 wk. Liver and plasma vitamin E were increased in mice fed the vitamin A-free diet. Superoxide dismutases, catalase and Se-glutathione peroxidase were determined in dorsal skin homogenates, as well as the concentration of reduced glutathione (GSH) and of thiobarbituric acid-reactive substances (TBARS); the latter is an index of peroxidation of murine skin cell membranes. Vitamin A deficiency did not alter enzyme activities but enhanced the skin reserve of GSH, which appeared to be the reason for a decrease in endogenous lipid peroxidation. Statistical analysis showed a highly significant negative correlation ( R 2 > 0.6) between the concentrations of TBARS and GSH in these untreated animals. GSH could play a critical role in maintaining a lower background of lipid alteration in the skin of healthy animals and minimizing individual risk.