[Identification of genes regulated by HBV preS1-transactivated protein 2 binding protein 1].

Objective To identify genes regulated by HBV preS1-transactivated protein 2 binding protein 1(PS1TP2BP1).Methods PS1TP2BP1 gene was amplified by polymerase chain reaction (PCR)technique and cloned into the eukaryotic expression vector pcDNATM3.1/my-c-His A.The mRNAs isolated from HepG2 cells transfected recombinant eukaryotic expression vector pcDNATM3.1/mycHisA-PS1TP2BP1 and pcDNATM3.1/myc-HisA empty vector were used to construct subtractive library.The differentially expressed genes were idenfied and analyzed.Results 35 differentially expressed clones were obtained.Colony PCR identified 15 clones with 200-1000 bp inserts.Sequence analysis identified 1 5 differentially expressed genes.Conclusion This study provides data for further characterize the function of PS1TP2BP1. Key words: Hepatitis B virus;  Cloning, molecular;  Suppression subtractive hybridization;  PS1TP2BP1