In-Vitro Evaluation of Antioxidant Activity and Total Phenolic Content of Methanolic Extract of Convolvulus pluricaulis.

Cellular damage caused by reactive oxygen species (ROS) has been implicated in several diseases, and hence natural antioxidants have significant importance in human health. Convulvulus pluricaulis is an important traditional medicinal plant, the present study was carried out to determine the total phenolic content and antioxidant properties of methanolic extract of leaves of this plant. The total phenolic content for 50, 100 and 200 µg/ml extracts was 0.2092, 0.2380 and 0.3608 mg GAE/ gram. The radical scavenging activity of methanolic extract of Convolvulus pluricaulis and the standard was found to be highest at 100ug/ml which was 52.56% and 93.48% respectively. The concentration of Convolvulus pluricaulis needed for 50% inhibition (IC50) was found to be 90.56 μg/ml whereas 29.02μg/ml needed for BHT. Based on the results it can be concluded that methanol extract of Convulvulus pluricaulis, a natural herb may have potential antioxidant effects against several oxidants.