ShortCommunication Isolation andShort TermCulture ofCanine Adrenal Microvascular Endothelial Cells

ABSTRACTA rapid method for the isolationof endothelial cells from canineadrenal glands is described. Thetechnique is based on collagenasedigestion of adrenal glands follow-ing mechanical tissue disruption.The endothelial cells attach andspread onto plastic petri dishescoated with a commercial attach-ment factor approximately onehour after seeding. Duration tofirst passage was approximately one week. Doubling times were2-3days. Cells werepositively iden-tified with immunoperoxidasestaining for von Willebrand factorantigen.RESUMEUne methode rapide pour isolerles cellules endothe'liales 'a partirdes glandes surrenales de chien est decrite. Cette technique est basse sur le bris mecanique des glandessurrenales suivi d'une digestion parla collage'nase. Les cellules endo-the'liales adh'erent et s'etalent 'a lasurface deplat depetri en plastique enduit d'un facteur d'attachementcommercial une heure environapresmise en plaque. Le de'lai avant