Effect of recipient mouse splenocytes taken orally by donor mouse on GVHD after splenocyte transplantation

This study was aimed to explore whether the GVHD in mice can be ameliorated and the GVL effect in mice can be reserved by transfusion of lymphocytes of donors fed with recipient splenocytes effect.Male(DBA-2)mice(H-2d)as donors were fed with BALB/c splenocytes,DBA-2 splenocytes,bovine serum albumin,or regular chow,every other day.Induction of tolerance was assessed by a mixed lymphocyte reaction(MLR).Female(BALB/c)mice(H-2d)as recipients received total body irradiation(TBI)of 6.0 Gy(60Coγ-ray)followed by inoculation of 3×103 P388 mouse leukemia cells on the same day.Subsequently,tail vein injection of 2×107 splenocytes supplied by DBA-2 was undertaken.Control groups were fed identically without leukemia cell inoculation.The results showed that GVHD was significantly ameliorated and CD4+/CD8+ ratio increased in recipient-mice transplanted with splenocytes of tolerated dornors,compared with control animals.There was no significant difference in survival rate between different groups of recipients inoculated with leukemia cell.It is concluded that the peroral recipient-mouse splenocytes can ameliorate GVHD without hampering effect on GVL.