Comparison of Q fever serology methods in cattle, goats, and sheep

Coxiella burnetii is an obligate intracellular bacterium that is responsible for the zoonotic disease Q fever. The distribution of this agent is worldwide except for New Zealand, and infection can be asymptomatic in both human beings and animals. Chronic exposures can produce abortions, stillbirths, and infertility issues in animals and endocarditis in human beings. A commercial enzyme-linked immunosorbent assay (ELISA) kit marketed in the European Union was purchased to compare C. burnetii antibody detection methods. The current study examined the agreement of ELISA and complement fixation results in over 668 diagnostic ruminant sera submitted to the National Veterinary Services Laboratories for Q fever serologic testing. The majority of combined sera (548) were negative on both tests. Fifty-seven of the combined sera were positive on both tests. There were 45 combined sera with low complement fixation titers at 1:10 and negative ELISA results. The results were surprising given the expectations that ELIS...