Culturing rhodotorula glutinis in fermentation-friendly deep eutectic solvent extraction liquor of lignin for producing microbial lipid

Currently, deep eutectic solvents (DES) have attracted increasing attention due to their excellent performance in delignification. However, few studies focused on the treatment of DES waste liquid after extraction of lignin. In this work, the fermentation-friendly DES comprised of glycerol, choline chloride (ChCl) and acetic acid (AA) was applied for delignification of lignocellulose. Subsequently, the extraction effects of different DES were investigated, and the DES extraction liquor was used for lipid production. Results shows ChCl made little difference to lipid synthesis, while excessive AA exerted inhibitory effect on the growth of cells. Following pretreatment, the delignification exceeded 63%. When the DES liquid obtained after lignin extraction was used to produce lipid, the delay period was obvious, while the lipid yield and content were unaffected. Not only is the DES prepared in this study effective in delignification of lignocellulose, it is also applicable as raw material to produce lipid.