Use of HP selective medium to detect Helicobacter pylori associated with other enteric bacteria in seawater and marine molluscs

Aims:  This project investigated the utility of HP selective medium to isolate H. pylori cells from seawater and from marine molluscs. Methods and Results:  Nested-PCR was performed to reveal the presence of Helicobacter genus. All samples were cultured in HP selective medium and 16 cultures were initially selected as putative Helicobacter. Helicobacter spp. DNA were detected in 9/16 cultures and three of them had 99–100% homology to H. pylori based on 16S RNA gene sequence. Helicobacter pylori isolation was unsuccessful. On the basis of 16S RNA gene sequences the contaminating organisms were shown to be Proteus mirabilis and Vibrio cholerae. Conclusions:  These results indicate the coexistence of three predominant bacterial genera in the cultures and that HP selective medium can grow other enteric bacteria besides Helicobacter. Additional assays will improve the HP selective medium formulation for marine samples avoiding P. mirabilis and V. cholerae interferents. Significance and Impact of the Study:  This work shows the effectiveness of the selective HP medium for the Helicobacter culture from marine samples.