Nano-biocatalyst: Bi-functionalization of Protease and Amylase on Copper Oxide Nanoparticles

Abstract There are reports that co-immobilization of enzymes on solid supports can circumvent the problem of loss of enzyme activity in a soup of enzymes. To understand the mechanistic pattern by which solid support can ensure enzyme stability. Copper oxide nanoparticles (CuO Nps) were employed to immobilize protease and amylase. These are enzymes widely used together and at different stages in various industrial activities such as laundry, leather processing, etc.. The immobilization of enzymes was confirmed through FTIR, TGA, and zeta potential analysis. Enzyme activity assays were carried out to understand enzyme activity with and without immobilization. The interaction between the nanoparticle surface and enzyme was studied through molecular docking studies. Thus, the study provides an insight into how the immobilization of enzymes on nanoparticles could be beneficial for industrial applications.