Mechanisms of increased mitochondria-dependent necrosis in Wiskott-Aldrich syndrome platelets

Wiskott-Aldrich syndrome is associated with thrombocytopenia of unclear origin. We investigated real-time cytosolic calcium dynamics, mitochondrial membrane potential and PS exposure in single fibrinogen-bound platelets using confocal microscopy. The Wiskott-Aldrich syndrome platelets had higher resting calcium levels, more frequent spikes, and their mitochondria more frequently lost membrane potential followed by the PS exposure (in 22.9% of platelets vs 3.9% in controls, P