[Effect of chemotherapy with cisplatin and rapamycin against Hep-2 cells in vitro].

Objective To evaluate the effect of combined use of rapamycin and cisplatin in the chemotherapy of Hep-2 cells in vitro.Methods The inhibitory effects of rapamycin and cisplatin, used alone or in combination, on the proliferation of Hep-2 cells were measured with MTT assay and median-effect plot analysis.The cell cycle changes after the treatment were analyzed using flow cytometry and Hoechst 33258 immunofluorescence staining.Results The IC50 of rapamycin and cisplatin for inducing growth arrest of Hep-2 cells was 11.03 nmol/L and 8.81 μmol/L, respectively.Rapamycin alone caused cell cycle arrest of the Hep-2 cells in G1 phase.Rapamycin and cisplatin showed synergistic effects in the chemotherapy of Hep-2 cells(q1.15, King's Formula), causing significantly increased apoptosis ratio and growth inhibition rate of Hep-2 cells.Conclusion Combined use of rapamycin and cisplatin significantly improves the chemotherapeutic effect against Hep-2 cells.