Effects of megsin gene transfection on inflammation in diabetic mouse kidney and its mechanism

To observe the effects of megsin gene transfection on the expression of monocyte chemoattractant protein-1(MCP-1) and intercellular adhesion molecule-1(ICAM-1),and investigate the role of megsin in the pathogenesis of diabetic nephropathy,we established a model of diabetic mouse.Randomly selected 10 mice as the diabetic group(group B).The remaining 30 mice were respectively injected pCMV·SPORT6.1(group C),Megsin-pCMV·SPORT6.1(group D) via tail vein once a week,and the normal control group was established(group A).All animals were sacrificed at week 4 and kidney tissues were harvested.The expression levels of megsin,MCP-1,and ICAM-1 were detected by immunohistochemistry and Western blot.Electron microscopy was used to observe the pathological changes.In diabetic mouse kidney,we observed the increased expression of megsin,MCP-1,and ICAM-1,irregular thickening of glomerular basement membrane,mesangial expansion,and fusion of foot processes.The above changes were more significant after megsin gene transfection.Our results suggested that megsin gene can up-regulate the expression of MCP-1 and ICAM-1,induce mesangial cell proliferation and mesangial extracellular matrix accumulation,which is probably one of the mechanisms of accelerating glomerulosclerosis.