The purification of SP1-β (pregnancy-specific β1-glycoprotein) using immobilized triazine dyes

Abstract Pregnancy-specific β 1 2 -glycoprotein was purified to 99% purity by sequential chromatography on carboxymethyl-cellulose (to remove other pregnancy-specific β 1 -glycoprotein variants), Cibacron blue 3G-A-Matrex Gel, Procion turquoise MX-G-Matrex Gel, Procion red H-8BN-Matrex Gel, and rabbit anti-normal human male serum-Matrex Gel. The purification was rapid, large-scale, and gave a yield of pregnancy-specific β 1 -glycoprotein of 35.1%.