[Effect of curcumin on expression of AKT and p-AKT in hippocampus CA1 area of App/PS1 double transgenic mice].

Objective: To observe the effect of curcumin on the expressions of AKT( serine-threonine kinase,AKT,also known as PKB) and p-AKT( phosphated serine-threonine kinase,p-AKT) in APP / PS1 double transgenic mice of the AD model.Method: Three-month-old APP /PS1 double transgenic mice were randomly divided into the model group,the rosiglitazone( 10 mg·kg- 1·d- 1) group,and high( 400 mg·kg- 1·d- 1),medium( 200 mg·kg- 1·d- 1) and low( 100 mg·kg- 1·d- 1) dose curcumin groups. Non-transgenic mice of the same age and background were selected as the control group( n = 12). After all of the six groups were intragastrically administered for consecutively three months,the protein expressions of AKT and p-AKT in hippocampus CA1 area were detected by immunohistochemistry and Western blot. Result: The results of immunohistochemistry showed that the expression of AKT and p-AKT positive cells in hippocampus CA1 area significantly decreased in the model group( P 0. 05 and P 0. 01). Compared with the model group,AKT and p-AKT positive cells of hippocampus CA1 area increased obviously in the rosiglitazone group and high and medium dose curcumin group( P 0. 05 or P 0. 01),especially the medium dose group( P 0. 01). The results of Western blot were consistent with that of immunohistochemistry. Conclusion: Curcumin can recover the decreased AKT and p-AKT cells in hippocampus CA1 area of APP / PS1 double transgenic mice of the AD model,suggesting that curcumin may regulate AKT and its phosphorylation process,as well as PI3K / AKT insulin signal transduction pathway,and show the anti-AD effect.