Signalling mechanisms of SDF-induced endothelial cell proliferation and migration.

Abstract The aim of our study was to investigate the effect of stromal-derived factor-1-α (SDF-1-α) on endothelial angiogenic effects. SDF-1-α (50 ng/ml) increased the number of cultured endothelial cells from 33,653 ± 1183 to 55,398 ± 2741, which significantly reduced by adding the BK Ca -inhibitor iberiotoxin, or the endothelial nitric oxide synthase-blocker, l -NMMA ( n  = 24, p l -NMMA ( n  = 12, p Ca open-state probability (NPo) was analysed using the patch-clamp technique and NPo was increased from 0.003 (control) to 0.052 (SDF-1-α; n  = 10, p l -NMMA and significantly reduced by iberiotoxin ( n  = 15, p Ca and an increased production of NO.