Mise en évidence ?amines liées à des structures cellulaires chez Nicotiana tabacum et Lycopersicum esculentum

Demonstration of amines connected with cellular structures in Nicotiana tabacum and Lycopersicum esculentum. The level of free and bound amines in various tissues of Nicotiana tabacum cv. Xanthi was investigated. After acid hydrolysis of different pellets from ethanol or buffer extractions, three aliphatic polyamines (putrescine, spermidine, spermine) and one aromatic monoamine (tyramine) were found in different proportions according to the nature of the tissue. Roots contain high quantities of insoluble tyramine bound very strongly to cellular structures. On the contrary, there were only traces of bound tyramine in leaves and stem. After extraction and purification of cell structures, tyramine was shown to be bound to one component of the cell wall. Addition of pronase or cellulase to the extract did not break the tyramine complex. In tobacco roots, insoluble tyramine concentration increased with plant development. High content of bound tyramine was also found in Lycopersicum esculentum roots. The possibility that tyramine binds with lignin is discussed.