In vitro effects of 635 nm low intensity diode laser irradiation on the fat distribution of one adipose cell

In vitro effects of 635-nm diode laser irradiation on the lipidic inclusions and the cellular fat distribution were observed in situ on a selected multilocular adipose cell in culture by an effective laser power of 3.16 mW. Selected microscopic field was 12 times sequentially irradiated, using 100 seconds exposures, a free spot of 5 mm and effective energy density of 1.6 Jcm -2 per exposure. Same field was irradiated 24 times using a beam spot of 10 mm, 0.4 Jcm -2 . Digital microphotograph sequences permit to observe and follow changes in fat distribution. Results show changes in fat vesicles. Microscopic follow-up shows an almost empty vacuole 15 hours after irradiation, the cell was empty after 42 hours, and dies after 52 hours.