Research on Ulinastatin's Mechanism of Immunity in Anti-aging

Objective: To study the anti-aging role of ulinastatin in mice.Methods: The senescent mice models were made and divided randomly into control group and treatment group,and were injected with normal sodium and ulinastatin respectively.After three weeks,the levels of SOD,MDA,IL-6 and IL-8 in the mice serum were detected by ELISA.The TI and SI were measured,and CD4~(+)T cells and CD8~(+)T cells were counted by flow cytometry.Results: In the treatment group,the level of SOD in the serum of mice was highly increased and the levels of MDA,IL-6 and IL-8 were decreased.The TI was increased,while the SI never changed.The amount of CD4~(+)T cells did not change significantly,while the amount of CD8~(+)T cells was increased and the ratio of CD4~(+)/CD8~(+)T cells were decreased.Conclusion: Ulinastatin might resist the lipid peroxidation which was caused by free radicals and its products of metabolism,might delay the process of atrophy of thymus gland,and increase the amount of T cells as well.The experiment showed that the ulinastatin might have the function of anti-aging.