台灣地區根腐線蟲Pratylenchus coffeae（Nematoda: Pratylenchidae）之鑑定

Eight populations of root-lesion nematode were isolated from the roots of sweet orange (Citrus sinensis), tankan (C. tankan), buntan (C. grandis f. buntan), lemon (C. limon), avocado (Persea americana), grape (Vitis vinifera) and the yam tubers using modified Baermann funnel method in Taiwan during October 2007 to March 2011. Morphological features observed via optical microscopy and SEM showed that the 8 nematode populations have low and flat lip region, with two annules; the labial disc and labial plate of the lip region are integrated into a smooth face and most of end-tail shapes are truncate, few are bluntly pointed. Based on the morphology, morphometrics and ribosomal DNA fragment sequences, including full 5.8S gene, ITS-1, ITS-2 and partial 18S and 28S gene, we have identified the 8 populations of root-lesion nematode as Pratylenchus coffeae (Zimmermann, 1898) Filipjev & Schuurmans Stekhoven, 1941. The length of representative rDNA fragment sequence of P. coffeae was 1249 bp, in which the lengths of ITS-1, ITS-2 and 5.8S gene were 484 bp, 311 bp and 157 bp, respectively.