Hydrophobic charge induction chromatography:principles, characteristics and applications

Hydrophobic charge induction chromatography(HCIC)is a new kind of chromatographic method developed in recent years. The HCIC ligands normally combine the hydrophobic and electrostatic interactions,thus HCIC belongs to a kind of mixed-mode chromatography. HCIC has been widely studied for protein separation,especially for antibody purification,which shows good operation and separation selectivity. This review focuses on the principles,characteristics and applications of HCIC,which would be helpful for the future researches and applications.