P-450 hemeproteins of Rhizobium japonicum purification by affinity chromatography and relationship to P-450CAM and P-450LM-2☆

Abstract Rhizobium hemeproteins P-450 a, b , and c cross react with antibodies to P-450 CAM and P-450 LM-2 . Anti P-450 CAM IgG and phenobarbital, each bound to Sepharose 4B, were effective in purification of Rhizobium P-450 c ; the latter was more convenient. The amino acid composition of highly purified Rhizobium P-450 c resembles the compositions of P-450 CAM and P-450 LM-2 . These results suggest that P-450 heme proteins of unrelated substrate specificities may nevertheless contain similar structural features.