Representative appressorium stage cDNA library of Magnaporthe grisea

A mature appressorium cDNA library of rice blast fungus, Magnaporthegrisea, was constructed in a λ TriplEx2 vector by SMARTTM cDNA library containing 2.37×10^6 independent clones about 100% of which harbor foreign cDNA inserts with average size of 660 bp. Of 9 randomly selected clones, 2 expressed sequence tags (ESTs) sequences did not have homologous EST sequences of M. grisea in Gen Bank. The appressorium cDNA library is suitable for gene expression analysis and function analysis of the late stages of appressorium formation and the early stages of penetration of M. grisea.