Simultaneous Determination of Seven Synthetic Colorants in Wine by Dispersive Micro-Solid-Phase Extraction Coupled with Reversed-Phase High-Performance Liquid Chromatography

A novel and effective dispersive micro-solid-phase extraction (d-mSPE) using ethanediamine-functionalized magnetic Fe3O4 polymer (EDA-MP) as an efficient adsorbent in wine sample was developed. Based on this, a simple and time-saving analytical method for the simultaneous determination of seven synthetic colorants (i.e., tartrazine, amaranth, carmine, sunset yellow, allura red, brilliant blue and erythrosine) in wine by reversed-phase high-performance liquid chromatography with an ultraviolet detector was established. The experimental parameters, including the chromatographic retention behavior of studied synthetic colorants, the effect of the usage amount of cross-linking monomer, the effect of the usage amount of EDA-MP on the recovery and the recyclability of the adsorbents, were studied in detail. The results showed that the EDA-MP could be reused efficiently at least six times. Under optimized conditions, the recoveries for all analytes were in the range of 88.6–105.2%, with the intraday relative standard deviations (RSDs) ranging from 2.1 to 8.2% and the interday RSDs ranging from 3.4 to 8.7%, and all the analytes had good linearities in the tested ranges with correlation coefficients (r) >0.9995. The limits of quantification for seven synthetic colorants were between 0.12 and 0.45 mg L. The developed method was successfully applied to wine samples, and it was confirmed that the EDA-MP particles were highly effective d-m-SPE materials.