Molecular biology studies of protein synthesis and 45Ca transport in striated and cardiac muscle of rabbits with experimental glandulardys functions.

The aim of our study was related with investigation of hormone excess treatment (Thyroxine and Hydrocortisone) upon heart and skeletal muscle metabolism, looking for modifications in nucleic acid and protein synthesis by means of radioisotope methods of 45Ca,3H Tryptohane and 3H Uridine uptake. In young heart rabbits treated with 0.625mg Hydrocortisone, a reduction in 45Ca uptake has been recorded ,while in old rabbits there is a progressive increase in 45Ca uptake as a function of dose of hormone used. As far as skeletal muscle is concerned, both in young and old rabbits treated with Hydrocortisone there is a progressive decline in cellular receptors for 45Ca affinity. An increase in the uptake of 3H Uridine for 0.15 and 1.50 mg thyroxine in rabbit heart has been recorded while at 0.75mg there is a decrease in the uptake. In skeletal muscle, there is a progressive increase in RNA synthesis under hormone impulse as a function of admitted dose in comparison with Controls.