Preparation of Tritium-Labeled Hyaluronic Acid by Tritium Thermal Activation Method

The principal possibility for preparing tritiated hyaluronic acid by the thermal activation method is demonstrated. The influence exerted by the conditions of preparing substances and the conduction of the isotope exchange reaction on the specific activity of the labeled product and the uniformity of tritium distribution among the molecules with different molecular weights is determined. It is shown that under mild experimental conditions and double dialysis purification of the samples (before and after the introduction of tritium), tritium-labeled hyaluronic acid with a specific activity of 26–52 GBq/g can be obtained without significant changes in the molecular weight distribution.