First Derivative Synchronous Spectrofluorimetric Determination of Cyproheptadine Hydrochloride in Presence of its Oxidative Degradation Product at Critical Micelle Concentration

Simple and sensitive first derivative synchronous spectrofluorimetric method was developed for the determination of cyproheptadine hydrochloride in presence of its oxidative degradation product. The method was based on measuring the synchronous fluorescence of the drug in water at Δλ of 120 nm in the presence of a sodium dodecyl sulphate as a micellar system. The peak amplitude of the first derivative spectra was measured at 418 nm for the drug. The different experimental parameters affecting the synchronous fluorescence intensity of CYP were studied and optimized. The peak amplitude–concentration plot was rectilinear over the range of 0.1–1.6 µ gmL−1. The limit of detection (LOD) was 0.02 and quantification limit (LOQ) was 0.06 µgmL−1. The proposed method was successfully applied to commercial tablets. Statistical comparison of the results with those of the official method revealed good agreement and proved that there was no significant difference in the accuracy and precision of the two methods.