Method for simultaneously separating high-purity phycocyanin and allophycocyanin and application thereof

The invention discloses a method for simultaneously separating high-purity phycocyanin and allophycocyanin and application thereof. The method comprises the following steps of: repeatedly freezing and thawing spirulina dry powder, ultrasonically crushing, and centrifuging, wherein supernate is a crude phycobiliprotein extracting solution; then adding ammonia sulfate, and taking out the supernate when the saturation degree is 25-35%; then, adding ammonia sulfate, taking out precipitates when the saturation degree is 60-70%, dialyzing for precipitation to obtain a primarily purified phycobiliprotein extracting solution, and loading the primarily purified phycobiliprotein extracting solution to a hydroxyapatite column; and collecting 0.02-0.03M phosphate buffered solution which contains 0.1-0.2M sodium chloride and has the pH value of 6.5-7.2, and 0.1-0.12M phosphate buffered solution which contains 0.1-0.2M sodium chloride and has the pH value of 6.5-7.2, wherein the 0.02-0.03M phosphate buffered solution is phycocyanin and the 0.1-0.12M phosphate buffered solution is allophycocyanin. The method disclosed by the invention can be used for simultaneously preparing phycocyanin with a purification factor reaching 4.5 and allophycocyanin with a purification factor reaching 3 as well as analogues containing selenium and tellurium, and the purification scale of the method is amplified so as to acquire high-purity protein of a gram level through primary chromatography, so that the purification cost is greatly reduced, and the method can be used for producing the phycocyanin and the allophycocyanin.