Peranan Fruktosa, Rafi nosa, dan Trehalosa pada Kriopreservasi

The objective of this study was to evaluate the effectiveness of carbohydrates supplementation on the stallion semen cryopreservation. Semen was collected from 3 stallions using artifi cial vagina twice a week. Collected semen was evaluated macroand microscopically. Semen showed >60% progressive motility was then divided into 3 tubes and diluted with skim milk 1:1, centrifuged at 1006 g for 10 minutes. Supernatan was removed and each pellet rediluted either with skim milk extender supplemented with 50 mM trehalose (ST); 50 mM raffi nose (SR) or 100 mM fructose (SF) with the concentration of sperm were 200x10 6 ml -1 . Extended semen packed into minitub 0.3 ml and equilibrates at 4 o C for 2 hours, freezes in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196 o C for further evaluation. After 24 hours, the semen was thawed at 37 o C for 30 second. The results of this experiment indicated that trehalose supplementation in skim milk was found to signifi cantly improve the percentage of sperm motility (P<0.05) for stallion 1 and 3 compared to raffi nose and fructose. But in all stallions, trehalose and fructose were superior compared to raffi nose.