OBTAINING OF ANTIOXIDANT PEPTIDE FROM BOVINE PLASMA HYDROLYSATES AND EFFECT OF THE DEGREE OF HYDROLYSIS ON ANTIOXIDANT CAPACITY OBTENCI ´ ON DE P´ EPTIDOS ANTIOXIDANTES DE HIDROLIZADOS DE PLASMA DE BOVINO Y EFECTO DEL GRADO DE HIDR ´ OLISIS EN SU CAPACIDAD ANTIOXIDANTE

In recent years there has been increased the interest in evaluate antioxidative bioactive peptides derived from food sources, bovine plasma have been shown to possess high antioxidant activity. Oxygen radical antioxidant capacity (ORAC) and trolox equivalent antioxidant capacity (TEAC) assays were compared in order to estimate the antioxidant capacity of bovine plasma hydrolysates (BPH) obtained for Alcalasefi 2.4 L. The e ect of the degree of hydrolysis (DH) was also evaluated for such activity. Additionally, the peptide fractions with the greatest activity were isolated by using Ultrafiltration Membranes, Ion Exchange Chromatography, and Reverse-Phase High Performance Liquid Chromatography (RP-HPLC). An increase of the degree of hydrolysis was found for these methods that correspond to an increase in the antioxidant activity; besides, the high relationship between ORAC and TEAC assays, with a correlation coe cient of 0.974, fitted a double squared model. A peptidic fraction with highest antioxidant capacity was obtained by separation processes, with values 4457.9 72.5 and 1409 43.41 molTE/g for ORAC and TEAC methods respectively, and contained peptides with sequences