[Regulating effect of anodonta glucan HBP-A on chondrocytes through Wnt pathway].

Objective:To investigate regulation function of anodonta glucan HBP-A on chondrocytes through Wnt pathway in vitro. Methods:Rat chondrocytes were cultured and differentiated induced with IL-1β(10 ng / ml) in vitro. Chondrocytes were divided into five groups:IL-1βgroup,IL-1β+IWP-2(5 μM,Wnt pathway inhibitor) group,IL-1β+ HBP-A(0.3mg / ml) group and IL-1β+IWP-2 +HBP-A group. Wnt-3a,β-catenin(24 h,48 h,72 h) and MMP-13(72 h) genes expression were detected by Rt-PCR,while β-catenin,MMP-13,Sox-9 and coll-Ⅱ(48 h) protein expression were measured by Western-blot. Results:After induction of IL-1β,gene expression of Wnt-3a,β-catenin and MMP-13 were increased,so were the protein expression of β-catenin and MMP-13. In contrast,protein expression of Sox-9 and Coll-Ⅱwere declined. Following addition of HBP-A,Wnt-3a,β-catenin and MMP-13 were shown as induction of IL-1β,but protein expression of Sox-9and Coll-Ⅱwere upgraded. Combining HBP-A with IWP-2 led to the lowest level in Wnt-3a,β-catenin gene and β-catenin protein expression and highest expression of Sox-9 protein. Conclusion:HBP-A could not only delay the differentiation of chondrocytes through downgrading the signal expression of Wnt / β-catenin,but also adjust the expression of Wnt-3a,β-catenin and Sox-9 when combinated with the Wnt inhibitor.